Methylation: Methionine is first enzymatically converted to S-adenosylmethionine (SAM), the principal methyl donor for methylation of DNA, RNA, protein, phospholipids, creatinine and neurotransmitters. S-adenosylhomocysteine (SAH) is generated as a product of all SAM-dependent methylation reactions and is hydrolyzed to homocysteine (Hcy) and adenosine through a reversible reaction (AHCY). SAH is a potent inhibitor of all SAM-dependent methylation reactions. Efficient removal of adenosine and Hcy is imperative to prevent accumulation of SAH. The enzymatic activities of adenosine kinase, adenosine deaminase and 5'-nucleosidases contribute to maintaining normal levels of adenosine.

Transmethylation: Hcy is normally primarily removed or recycled by remethylation to methionine through a series of reactions that require 5-methyltetrahydrofolate, B12 and betaine to complete the normal methionine cycle. A low ratio of SAM to SAH is a sensitive indicator of under-methylation. elevated plasma Hcy is an independent risk factor for cardiovascular disease (CVD). Recent research suggests that elevated SAH may be an even better predictor of risk for CVD.

Transsulfuration: Methionine > Homocysteine > Cysteine The methionine transsulfuration pathway occurs primarily in the liver and kidneys, and diverts Hcy away from remethylation to methionine toward synthesis of conditionally essential amino acid cysteine, essential sulfate, taurine and glutathione. Homocysteine in the presence of serine and B6 is enzymatically converted to cystathionine and ultimately cysteine. Cysteine is the rate-limiting amino acid in the biosynthesis of quintessential glutathione (GSH). GSH is pivotal in the regulation of intracellular redox homeostasis, oxidative stress, immune function, DNA synthesis and repair. apoptosis and detoxification of metals and chemicals. The DDI Methylation Profile evaluates the plasma levels of methionine, cysteine, SAM, SAH, Hcy, adenosine and cystathionine, and provides the important "methylation index," a ratio of SAM to SAH.
The test results can facilitate appropriate individualized interventions to improve or normalize methionine metabolism and ameliorate or prevent adverse consequences associated with inadequate methylation and/or transsulfuration capacity.

Analytes

• Cystathionine

• Cysteine

• Homocysteine

• Methionine

• s-adenosylhomocysteine;plasma

• s-adenosylmethionine; plasma

• SAH

• SAM

  14-day turnaround time for labs to produce your report. This test requires a full blood sample. Click here to find a phlebotomist in your area. All providers listed under "Find a blood draw" are wholly independent from Body Fabulous and their fees are not included within the test prices. Please note the cost of your blood draw is payable directly to your nurse provider (approx £40-£100). Please check before making your appointment that the clinic or provider offers centrifugation.

• This blood collection kit is posted to you from the UK and after you have arranged for your sample to be taken you simply post it back to the lab free of charge using the specialist UPS label provided. When we receive your results back from the labs we will email you your PDF report along with our functional medicine advice via email to support you.

• Within your test collection kit, we provide you with a free post delivery label and packing bag to return your completed specimen. Please take kit box and instructions to your chosen phlebotomist, and request a centrifuged sample. Please note you must arrange your phlebotomist appointment independently and pay your service provider directly. Book your blood to be drawn and collected Monday to Thursday only ensuring the next day is not a bank holiday or weekend in the UK. Note: On returning home from your phelbotomy appointment pop your bloods and the gel pack in the freezer and once you have arranged a time for UPS to collect you sample you can then package your sample up still frozen.

  Preparation • Fast for 12 hours before your blood test • Do not stop any medications. For 48 hours prior to the collection, discontinue taking dietary supplements containing methionine, cysteine, SAMe, and vitamins containing folate unless otherwise directed by your physician. Please only send samples back Mon-Thurs.

• Click HERE to view sample report

• Click HERE to view sample instructions